Preparation of stable Pd nanocubes and their use in biological labeling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381927" target="_blank" >RIV/60077344:_____/12:00381927 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/12:00381927 RIV/68378050:_____/12:00381927
Result on the web
<a href="http://dx.doi.org/10.1016/j.colsurfb.2012.05.023" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2012.05.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.colsurfb.2012.05.023" target="_blank" >10.1016/j.colsurfb.2012.05.023</a>
Alternative languages
Result language
angličtina
Original language name
Preparation of stable Pd nanocubes and their use in biological labeling
Original language description
Stable Pd nanocubes (PdNC) with the average size 15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Colloids and Surfaces B-Biointerfaces
ISSN
0927-7765
e-ISSN
—
Volume of the periodical
100
Issue of the periodical within the volume
1 December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
205-208
UT code for WoS article
000307683900029
EID of the result in the Scopus database
—