Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00367418" target="_blank" >RIV/60077344:_____/11:00367418 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/11:43883012 RIV/61389013:_____/11:00367418
Result on the web
<a href="http://dx.doi.org/10.1017/S1431927611000547" target="_blank" >http://dx.doi.org/10.1017/S1431927611000547</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S1431927611000547" target="_blank" >10.1017/S1431927611000547</a>
Alternative languages
Result language
angličtina
Original language name
Application of Colloidal Palladium Nanoparticles for Labeling in Electron Microscopy
Original language description
The application of palladium nanoparticles as electron-dense markers for labeling in both transmission and scanning electron microscopy requires their conjugation to a specific protein. The conjugation protocol described here includes the dihydrolipoic acid capping of Pd nanoparticles and their subsequent covalent attachment to protein molecules such as streptavidin, protein A, or avidin. The single-step reaction was mediated using the cross-linking agent ethyl-3-(3- dimethylaminopropyl)carbodiimide. The final Pd conjugates were fully functional, as demonstrated by labeling of ultrathin resin sections of either bovine serum albumin or secretory granules of the salivary gland isolated from the partially fed female Ixodes ricinus tick. The results were quantified, evaluated, and compared with results obtained using commercially available gold particle conjugates. Both the streptavidin-Pd conjugate and commercial streptavidin-Au conjugate were used to detect fucosylated glycans.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy and Microanalysis
ISSN
1431-9276
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
810-816
UT code for WoS article
000295609100020
EID of the result in the Scopus database
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