Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10371535" target="_blank" >RIV/00216208:11310/16:10371535 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/16:00461980 RIV/60076658:12310/16:43890612

Result on the web

<a href="http://dx.doi.org/10.1016/j.nano.2015.09.008" target="_blank" >http://dx.doi.org/10.1016/j.nano.2015.09.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nano.2015.09.008" target="_blank" >10.1016/j.nano.2015.09.008</a>

Result language

angličtina

Original language name

Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

Original language description

We present a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals. From the Clinical Editor: The use of transmission electron microscopy has become an indispensible tool in the detection of cellular proteins. In this short but interesting article, the authors described their new method of labeling and the identification of four different proteins simultaneously, which represents another advance in imaging technique.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30224 - Radiology, nuclear medicine and medical imaging

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nanomedicine: Nanotechnology, Biology, and Medicine

ISSN

1549-9634

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

105-108

UT code for WoS article

000371800600010

EID of the result in the Scopus database

2-s2.0-84957064578