Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F15%3A00449551" target="_blank" >RIV/68081731:_____/15:00449551 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s13594-015-0253-2" target="_blank" >http://dx.doi.org/10.1007/s13594-015-0253-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13594-015-0253-2" target="_blank" >10.1007/s13594-015-0253-2</a>

Result language

angličtina

Original language name

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Original language description

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allowfor precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BH - Optics, masers and lasers

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Dairy Science & Technology

ISSN

1958-5586

e-ISSN

—

Volume of the periodical

95

Issue of the periodical within the volume

5

Country of publishing house

FR - FRANCE

Number of pages

14

Pages from-to

687-700

UT code for WoS article

000361480900009

EID of the result in the Scopus database

—