Immunogold Labelling for Scanning Electron Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F10%3A00503596" target="_blank" >RIV/68378050:_____/10:00503596 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-60761-783-9_24" target="_blank" >http://dx.doi.org/10.1007/978-1-60761-783-9_24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-60761-783-9_24" target="_blank" >10.1007/978-1-60761-783-9_24</a>
Alternative languages
Result language
angličtina
Original language name
Immunogold Labelling for Scanning Electron Microscopy
Original language description
Scanning electron microscopes are useful biological tools that can be used to image the surface of whole organisms, tissues, cells, cellular components and macromolecules. Processes and structures that exist at surfaces can be imaged in pseudo or real 3D at magnifications of anything from about x10 to x1,000,000. Therefore a whole multicellular organism, such as a fly, or a single protein embedded in one of its cell membranes can be visualised. In order to identify that protein at high resolution, or to see and quantify its distribution at lower magnifications, samples can be labelled with antibodies. Any surface that can be exposed can potentially be studied in this way. Presented here is a generic method for immunogold labelling for scanning electron microscopy, using two examples of specimens: isolated nuclear envelopes and the cytoskeleton of mammalian culture cells. Various parameters for sample preparation, fixation, immunogold labelling, drying, metal coating and imaging are discussed so that the best immunogold scanning electron microscopy results can be obtained from different types of specimens.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods in Molecular Biology
ISSN
1064-3745
e-ISSN
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Volume of the periodical
657
Issue of the periodical within the volume
January
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
297-313
UT code for WoS article
000280523600024
EID of the result in the Scopus database
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