Fine structure of the "PcG body" in human U-2 OS cells established by correlative light-electron microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10482" target="_blank" >RIV/00216208:11110/11:10482 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4161/nucl.2.3.15737" target="_blank" >http://dx.doi.org/10.4161/nucl.2.3.15737</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Fine structure of the "PcG body" in human U-2 OS cells established by correlative light-electron microscopy

Original language description

Polycomb group (PcG) proteins of the Polycomb repressive complex 1 (PRC1) are found to be diffusely distributed in nuclei of cells from various species. However they can also be localized in intensely fluorescent foci, whether imaged using GFP fusions toproteins of PRC1 complex, or by conventional immunofluorescence microscopy. Such foci are termed Polycomb bodies (PcG bodies), and are believed to be situated into the nuclear intechromatin compartment. However, an ultrastructural description of the PcGbody has not been reported to date. To establish the ultrastructure of PcG bodies in human U-2 OS cells stably expressing recombinant polycomb BMI1-GFP protein, we used correlative light electron microscopy (CLEM) implemented with high-pressure freezing, cryosubstitution and on section labeling of BMI1 protein with immunogold. This approach allowed us to clearly identify fluorescent PcG bodies, not as distinct nuclear bodies, but as nuclear domains enriched in separated heterochromatin

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

<a href="/en/project/LC535" target="_blank" >LC535: Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleus

ISSN

1949-1034

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

219-228

UT code for WoS article

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EID of the result in the Scopus database

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