Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00461980" target="_blank" >RIV/60077344:_____/16:00461980 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/16:43890612 RIV/00216208:11310/16:10371535
Result on the web
<a href="http://dx.doi.org/10.1016/j.nano.2015.09.008" target="_blank" >http://dx.doi.org/10.1016/j.nano.2015.09.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nano.2015.09.008" target="_blank" >10.1016/j.nano.2015.09.008</a>

Result language
angličtina
Original language name
Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy
Original language description
We present a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
—

Project
<a href="/en/project/TE01020118" target="_blank" >TE01020118: Electron microscopy</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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