An advanced fast method for the evaluation of multiple immunolabelling using gold nanoparticles based on low-energy STEM
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604783" target="_blank" >RIV/60077344:_____/24:00604783 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/24:43908880 RIV/00216208:11310/24:10497659
Result on the web
<a href="https://doi.org/10.1038/s41598-024-60314-0" target="_blank" >https://doi.org/10.1038/s41598-024-60314-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-024-60314-0" target="_blank" >10.1038/s41598-024-60314-0</a>
Alternative languages
Result language
angličtina
Original language name
An advanced fast method for the evaluation of multiple immunolabelling using gold nanoparticles based on low-energy STEM
Original language description
We present a powerful method for the simultaneous detection of Au nanoparticles located on both sides of ultrathin sections. The method employs a high-resolution scanning electron microscope (HRSEM) operating in scanning transmission electron microscopy (STEM) mode in combination with the detection of backscattered electrons (BSE). The images are recorded simultaneously during STEM and BSE imaging at the precisely selected accelerating voltage. Under proper imaging conditions, the positions of Au nanoparticles on the top or bottom sides can be clearly differentiated, hence showing this method to be suitable for multiple immunolabelling using Au nanoparticles (NPs) as markers. The difference between the upper and lower Au NPs is so large that it is possible to apply common software tools (such as ImageJ) to enable their automatic differentiation. The effects of the section thickness, detector settings and accelerating voltage on the resulting image are shown. Our experimental results correspond to the results modelled in silico by Monte Carlo (MC) simulations.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
10150
UT code for WoS article
001284629600041
EID of the result in the Scopus database
2-s2.0-85191992924