Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00392817" target="_blank" >RIV/60077344:_____/13:00392817 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs00436-012-3244-8" target="_blank" >http://link.springer.com/article/10.1007%2Fs00436-012-3244-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00436-012-3244-8" target="_blank" >10.1007/s00436-012-3244-8</a>

Result language

angličtina

Original language name

Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

Original language description

From 2009 to 2011, the occurrence of Cryptosporidium spp. was investigated on 22 farms in the Czech Republic. A total of 1,620 individual faecal samples of pigs of all age categories (pre-weaned, starters, pre-growers, growers, and sows) were evaluated for presence of Cryptosporidium spp. by standard microscopy and molecular tools. Genotyping was done through PCR amplification and characterization of the SSU rRNA (species-specific protocols) and GP60 loci. Cryptosporidium spp. was found on 16 of 22 farms with a range 0.9?71.4 %. Overall, 194 (12 %) specimens were positive by microscopy and 353 (21.8 %) by PCR. While RFLP and direct sequencing of the PCRamplified products showed presence of Cryptosporidium suis (142), Cryptosporidium scrofarum (195), Cryptosporidium muris (3) and 13 samples had mixed infections with C. suis and C. scrofarum, species-specific molecular tools identified C. suis (224), C. scrofarum (208), Cryptosporidium parvum subtype IIa A16G1R1b (1), and C. muris (3). I

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LH11061" target="_blank" >LH11061: Diversity, biology and phylogeny of Cryptosporidium spp. parasiting in rodents</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology Research

ISSN

0932-0113

e-ISSN

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Volume of the periodical

112

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

1143-1154

UT code for WoS article

000315443100027

EID of the result in the Scopus database

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