Microsporidia and Cryptosporidium in horses and donkeys in Algeria: Detection of a novel Cryptosporidium hominis subtype family (Ik) in a horse

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00453391" target="_blank" >RIV/60077344:_____/15:00453391 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12510/15:43890144 RIV/60076658:12220/15:43890144
Result on the web
<a href="http://dx.doi.org/10.1016/j.vetpar.2015.01.007" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2015.01.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.vetpar.2015.01.007" target="_blank" >10.1016/j.vetpar.2015.01.007</a>

Result language
angličtina
Original language name
Microsporidia and Cryptosporidium in horses and donkeys in Algeria: Detection of a novel Cryptosporidium hominis subtype family (Ik) in a horse
Original language description
A total of 219 and 124 individual fecal samples of horses and donkeys, respectively, were screened for the presence of Cryptosporidium spp., Encephalitozoon spp., and Enterocytozoon bieneusi DNA by genus-specific nested PCR. Isolates were genotyped by sequence analysis of SSU rRNA, GP60, TRAP-C1, COWP, and HSP70 loci in Cryptosporidium, and the ITS region in microsporidia. Cryptosporidium spp. was detected on 3/18 horse farms and 1/15 farms where donkeys were kept. Overall, five (2.3%) horse and two (1.6%) donkey specimens were PCR positive for Cryptosporidium. Genotyping at SSU and GP60 loci revealed that three isolates from horses and donkeys were C. parvum subtype family IIaA16G1R1, one isolate from a horse was, C muris RN66, and one isolate from adonkey was C. muris TS03. An isolate from a horse shared 99.4% and 993% similarity with Cryptosporidium hominis and C. cuniculus, respectively, at the SSU locus. This isolate shared 100% identity with C. hominis at the TRAP-C1, COWP, and
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
<a href="/en/project/GA15-01090S" target="_blank" >GA15-01090S: Revealing Cryptosporidium diversity: Linking genetic variation to parasite biology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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