Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00392960" target="_blank" >RIV/60077344:_____/13:00392960 - isvavai.cz</a>

Alternative codes found

RIV/00023736:_____/13:00010694 RIV/60076658:12310/13:43885742

Result on the web

<a href="http://dx.doi.org/10.5504/BBEQ.2012.0141" target="_blank" >http://dx.doi.org/10.5504/BBEQ.2012.0141</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5504/BBEQ.2012.0141" target="_blank" >10.5504/BBEQ.2012.0141</a>

Result language

angličtina

Original language name

Transformation of tobacco cpDNA with fusion E7GGG/GUS gene and homologous recombination mediated elimination of the marker gene

Original language description

A transformation vector harboring the marker gene aadA conferring spectinomycin resistance and the fusion E7GGG/gus gene of interest was employed for the transformation of tobacco cpDNA via biolistics. The gene of interest consisted of the oncogenic E7GGG gene from human papillomavirus strain 16 fused with the reporter gus gene. We identified 6 plants harboring the E7GGG/gus gene only, where the marker gene was eliminated by homologous recombination, one plant with both transgenes, and one with the aadAgene only. RT-PCR showed the presence of the respective mRNAs in all 8 plants analyzed but Western blot proved that only the GUS part of the fusion E7GGG/GUS protein was present in all plants harboring the fusion gene.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/IAA500960903" target="_blank" >IAA500960903: Development of a Novel Recombinogenic Technique for Chloroplast Transformation and its Use for Production of human papillomavirus E7 Protein in Plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biotechnology & Biotechnological Equipment

ISSN

1310-2818

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

2

Country of publishing house

BG - BULGARIA

Number of pages

5

Pages from-to

3644-3648

UT code for WoS article

000317997400005

EID of the result in the Scopus database

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