Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43893693" target="_blank" >RIV/60461373:22330/12:43893693 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes

Original language description

The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemicalassay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

DK - Contamination and decontamination of soil including pesticides

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

5th International Symposium on Biosorption and Bioremediation

ISBN

978-80-7080-825-2

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

118-121

Publisher name

VŠCHT Praha

Place of publication

Praha

Event location

Praha

Event date

Jun 24, 2012

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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