Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012750" target="_blank" >RIV/60461373:22330/04:00012750 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cloning of a bacterial PCB-degrading gene into the plants of Nicotiana tabacum

Original language description

The target of this work is to increase biodegradation of polychlorinated biphenyls (PCBs) by the plants transformed with the gene from bacterial PCB degradation pathway. Bacterial aerobic PCB degradation pathway contains 4 steps (bph operon) in which thethird one (bphC) catalyzes the conversion of 2,3-dihydroxybiphenyl into 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid and thus destroyes the biphenyl ring. For this purpose the bphC gene encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was chosen from bacteria Pseudomonas testosteroni B-356 for cloning into plant Nicotiana tabacum. Different constructs in cassette with bphC containing genes for GFP (green fluorescent protein), GUS (glucuronidase) and LUC (luciferase) were prepared to allow sensitive detection of bphC expression in plants. Also construct in which bphC was joined to His tail was designed. The constructs with bphC/GFP was already transformed into the Nicotiana tabacum. The presence of bphC/GFP DNA and RNA was pro

Czech name

Klonování bakteriálních PCB degradujícícg genů do rostliny Nicotina tabacum

Czech description

Klonování bakteriálních PCB degradujícícg genů do rostliny Nicotina tabacum

Type

D - Article in proceedings

CEP classification

DK - Contamination and decontamination of soil including pesticides

OECD FORD branch

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Project

<a href="/en/project/LN00B030" target="_blank" >LN00B030: Center for molecular and gene biotechnologies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceeding of the 6th International Symposium and Exhibition on Environmental Contamination in Central and eastern Europe

ISBN

0-9748192-0-4

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

479

Publisher name

Florida State University

Place of publication

Florida

Event location

Praha

Event date

Sep 1, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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