Cloning of bacterial PCB degradative genes to plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012752" target="_blank" >RIV/60461373:22330/04:00012752 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/04:00017137

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Cloning of bacterial PCB degradative genes to plants

Original language description

The target of this work is to increase biodegradation of polychlorinated biphenyls (PCBs) by the plants transformed with the gene from bacterial PCB degradation pathway. Bacterial aerobic PCB degradation pathway contains 4 steps (bph operon) in which thethird one (bphC) catalyzes the conversion of 2,3-dihydroxybiphenyl into 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid and thus destroyes the biphenyl ring. For this purpose the bphC gene encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase was chosen from bacteria Pseudomonas testosteroni B-356 for cloning into plant Nicotiana tabacum. Different constructs in cassette with bphC containing genes for GFP (green fluorescent protein), GUS (glucuronidase) and LUC (luciferase) were prepared to allow sensitive detection of bphC expression in plants. Also construct in which bphC was joined to His tail was designed. The constructs with bphC/GFP was already transformed into the Nicotiana tabacum. The presence of bphC/GFP DNA and RNA was pro

Czech name

Klonování PCB degradujícího bakteriálního genu do rostlin

Czech description

Klonování PCB degradujícího bakteriálního genu do rostlin

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

—

Project

<a href="/en/project/LN00B030" target="_blank" >LN00B030: Center for molecular and gene biotechnologies</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Int. Biodeterioration and Biodegradation

ISSN

0964-8305

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

1

Pages from-to

247-248

UT code for WoS article

—

EID of the result in the Scopus database

—