Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00395286" target="_blank" >RIV/60077344:_____/13:00395286 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/13:00395286 RIV/60076658:12310/13:43885522 RIV/60109807:_____/13:#0000227
Result on the web
<a href="http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf" target="_blank" >http://link.springer.com/content/pdf/10.1007%2Fs12010-013-0325-9.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12010-013-0325-9" target="_blank" >10.1007/s12010-013-0325-9</a>
Alternative languages
Result language
angličtina
Original language name
Protease inhibitor from insect silk - activities of derivatives expressed in vitro and in transgenic potato
Original language description
Several recombinant derivatives of silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitorswere highly active against subtilisin and proteinase K. A construct of the gene was introduced into Agrobacterium tumefaciens that was then used for genetic transformation of the potato variety Velox. In vitro tests showed increased resistance to the late blight Phytophthora infestans in several transformed lines. No effect was seen on biological characteristics of Spodoptera littoralis indicating that the transformed potatoes may be harmless to non-target organisms.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QI91A229" target="_blank" >QI91A229: Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Biochemistry and Biotechnology
ISSN
0273-2289
e-ISSN
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Volume of the periodical
171
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
209-224
UT code for WoS article
000324109800018
EID of the result in the Scopus database
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