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Both human ferredoxins equally efficiently rescue ferredoxin deficiency in Trypanosoma brucei

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00396398" target="_blank" >RIV/60077344:_____/13:00396398 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/13:43885293

  • Result on the web

    <a href="http://dx.doi.org/10.1111/mmi.12264" target="_blank" >http://dx.doi.org/10.1111/mmi.12264</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/mmi.12264" target="_blank" >10.1111/mmi.12264</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Both human ferredoxins equally efficiently rescue ferredoxin deficiency in Trypanosoma brucei

  • Original language description

    Ferredoxins are highly conserved proteins that function universally as electron transporters. They not only require Fe-S clusters for their own activity, but are also involved in Fe-S formation itself. We identified two homologues of ferredoxin in the genome of the parasitic protist Trypanosoma brucei and named them TbFdxA and TbFdxB. TbFdxA protein, which is homologous to other eukaryotic mitochondrial ferredoxins, is essential in both the procyclic (=insect-transmitted) and bloodstream (mammalian) stage, but is more abundant in the active mitochondrion of the former stage. Depletion of TbFdxA caused disruption of Fe-S cluster biogenesis and lowered the level of intracellular haem. However, TbFdxB, which is present exclusively within kinetoplastid flagellates, was non-essential for the procyclic stage, and double knock-down with TbFdxA showed this was not due to functional redundancy between the two homologues. Heterologous expressions of human orthologues HsFdx1 and HsFdx2 fully resc

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Molecular Microbiology

  • ISSN

    0950-382X

  • e-ISSN

  • Volume of the periodical

    89

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    17

  • Pages from-to

    135-151

  • UT code for WoS article

    000320728800010

  • EID of the result in the Scopus database