Mitochondrial translation factors of Trypanosoma brucei: elongation factor-Tu has a unique subdomain that is essential for its function
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00420949" target="_blank" >RIV/60077344:_____/13:00420949 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/13:43885888
Result on the web
<a href="http://dx.doi.org/10.1111/mmi.12397" target="_blank" >http://dx.doi.org/10.1111/mmi.12397</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/mmi.12397" target="_blank" >10.1111/mmi.12397</a>
Alternative languages
Result language
angličtina
Original language name
Mitochondrial translation factors of Trypanosoma brucei: elongation factor-Tu has a unique subdomain that is essential for its function
Original language description
Mitochondrial translation in the parasitic protozoan Trypanosoma brucei relies on imported eukaryotic-type tRNAs as well as on bacterial-type ribosomes that have the shortest known rRNAs. Here we have identified the mitochondrial translation elongation factors EF-Tu, EF-Ts, EF-G1 and release factor RF1 of trypanosomatids and show that their ablation impairs growth and oxidative phosphorylation. In vivo labelling experiments and a SILAC-based analysis of the global proteomic changes induced by EF-Tu RNAidirectly link EF-Tu to mitochondrial translation. Moreover, EF-Tu RNAi reveals downregulation of many nuclear encoded subunits of cytochrome oxidase as well as of components of the bc1-complex, whereas most cytosolic ribosomal proteins were upregulated.Interestingly, T.brucei?EF-Tu has a 30-amino-acid-long, highly charged subdomain, which is unique to trypanosomatids. A combination of RNAi and complementation experiments shows that this subdomain is essential for EF-Tu function, but th
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP305%2F12%2F2261" target="_blank" >GAP305/12/2261: Characterization of the mitoproteome of the parasitic protist Trypanosoma brucei by means of recombinogenic engineering</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Microbiology
ISSN
0950-382X
e-ISSN
—
Volume of the periodical
90
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
744-755
UT code for WoS article
000330108000006
EID of the result in the Scopus database
—