Flow Sorting and Sequencing Meadow Fescue Chromosome 4F

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00422267" target="_blank" >RIV/60077344:_____/13:00422267 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/13:00422267

Result on the web

<a href="http://dx.doi.org/10.1104/pp.113.224105" target="_blank" >http://dx.doi.org/10.1104/pp.113.224105</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1104/pp.113.224105" target="_blank" >10.1104/pp.113.224105</a>

Result language

angličtina

Original language name

Flow Sorting and Sequencing Meadow Fescue Chromosome 4F

Original language description

The analysis of large genomes is hampered by a high proportion of repetitive DNA, which makes the assembly of short sequence reads difficult. This is also the case in meadow fescue (Festuca pratensis), which is known for good abiotic stress resistance and has been used in intergeneric hybridization with ryegrasses (Lolium spp.) to produce Festulolium cultivars. In this work, we describe a new approach to analyze the large genome of meadow fescue, which involves the reduction of sample complexity withoutcompromising information content. This is achieved by dissecting the genome to smaller parts: individual chromosomes and groups of chromosomes. As the first step, we flow sorted chromosome 4F and sequenced it by Illumina with approximately 503 coverage.This provided, to our knowledge, the first insight into the composition of the fescue genome, enabled the construction of the virtual gene order of the chromosome, and facilitated detailed comparative analysis with the sequenced genomes

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Physiology

ISSN

0032-0889

e-ISSN

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Volume of the periodical

163

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

1323-1337

UT code for WoS article

000326520900020

EID of the result in the Scopus database

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