Repetitive DNA: A Versatile Tool for Karyotyping in Festuca pratensis Huds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476564" target="_blank" >RIV/61389030:_____/17:00476564 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1159/000462915" target="_blank" >http://dx.doi.org/10.1159/000462915</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000462915" target="_blank" >10.1159/000462915</a>

Result language

angličtina

Original language name

Repetitive DNA: A Versatile Tool for Karyotyping in Festuca pratensis Huds

Original language description

FISH is a useful method to identify individual chromosomes in a karyotype and to discover their structural changes accompanying genome evolution and speciation. DNA probes for FISH should be chromosome specific and/or exhibit specific patterns of distribution along each chromosome. Such probes are not available in many plants including meadow fescue (Festuca pratensis Huds.), an important forage grass species. In the present study, various DNA repeats identified in Illumina shotgun sequences specific to chromosome 4F of F. pratensis were used as probes for FISH to develop the molecular karyotype of meadow fescue and to reveal a long-range molecular organization of its chromosomes. Five tandem repeats produced specific patterns on individual chromosomes. Their use in combination with probes for rRNA genes enabled the establishment of the molecular karyotype of meadow fescue. Most of the mobile genetic elements were dispersed along all the chromosomes except for the DNA transposon CACTA, which was localized preferentially to telomeric and subtelomeric regions, and a putative LTR element, which was localized to (peri)centromeric regions. Cytogenetic mapping of the 5 tandem repeats in other accessions of meadow fescue showed a highly similar distribution and confirmed the versatility and robustness of these probes.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytogenetic and Genome Research

ISSN

1424-8581

e-ISSN

—

Volume of the periodical

151

Issue of the periodical within the volume

2

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

96-105

UT code for WoS article

000404007600005

EID of the result in the Scopus database

2-s2.0-85016641443