Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00532642" target="_blank" >RIV/61389030:_____/20:00532642 - isvavai.cz</a>
Result on the web
<a href="http://doi.org/10.1016/j.cj.2020.02.008" target="_blank" >http://doi.org/10.1016/j.cj.2020.02.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cj.2020.02.008" target="_blank" >10.1016/j.cj.2020.02.008</a>
Alternative languages
Result language
angličtina
Original language name
Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat
Original language description
Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement. Owing to the limited number of probes available for fluorescence in situ hybridization (FISH), the resolution at which the karyotype of H. villosa can be characterized is poor, hampering accurate characterization of small segmental alien introgressions. We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H. villosa chromosome 6V (6VS). FISH with seven of them resulted in clear signals on H. villosa chromosomes. Using these, we constructed FISH karyotypes for H. villosa using oligo-6VS-1 and oligo-6VS-35 oligonucleotides and characterized the distribution of the two probes in five different H. villosa accessions. The new FISH probes can efficiently characterize H. villosa introgressions into wheat.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Crop Journal
ISSN
2095-5421
e-ISSN
—
Volume of the periodical
8
Issue of the periodical within the volume
4
Country of publishing house
CN - CHINA
Number of pages
6
Pages from-to
676-681
UT code for WoS article
000556540200016
EID of the result in the Scopus database
2-s2.0-85085209768