Long-range assembly of sequences helps to unravel the genome structure and small variation of the wheat–Haynaldia villosa translocated chromosome 6VS.6AL
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00549886" target="_blank" >RIV/61389030:_____/21:00549886 - isvavai.cz</a>
Result on the web
<a href="http://doi.org/10.1111/pbi.13570" target="_blank" >http://doi.org/10.1111/pbi.13570</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/pbi.13570" target="_blank" >10.1111/pbi.13570</a>
Alternative languages
Result language
angličtina
Original language name
Long-range assembly of sequences helps to unravel the genome structure and small variation of the wheat–Haynaldia villosa translocated chromosome 6VS.6AL
Original language description
Genomics studies in wild species of wheat have been limited due to the lack of references, however, new technologies and bioinformatics tools have much potential to promote genomic research. The wheat–Haynaldia villosa translocation line T6VS·6AL has been widely used as a backbone parent of wheat breeding in China. Therefore, revealing the genome structure of translocation chromosome 6VS·6AL will clarify how this chromosome formed and will help to determine how it affects agronomic traits. In this study, chromosome flow sorting, NGS sequencing and Chicago long-range linkage assembly were innovatively used to produce the assembled sequences of 6VS·6AL, and gene prediction and genome structure characterization at the molecular level were effectively performed. The analysis discovered that the short arm of 6VS·6AL was actually composed of a large distal segment of 6VS, a small proximal segment of 6AS and the centromere of 6A, while the collinear region in 6VS corresponding to 230–260 Mb of 6AS-Ta was deleted when the recombination between 6VS and 6AS occurred. In addition to the molecular mechanism of the increased grain weight and enhanced spike length produced by the translocation chromosome, it may be correlated with missing GW2-V and an evolved NRT-V cluster. Moreover, a fine physical bin map of 6VS was constructed by the high-throughput developed 6VS-specific InDel markers and a series of newly identified small fragment translocation lines involving 6VS. This study will provide essential information for mining of new alien genes carried by the 6VS·6AL translocation chromosome.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Biotechnology Journal
ISSN
1467-7644
e-ISSN
1467-7652
Volume of the periodical
19
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1567-1578
UT code for WoS article
000623502200001
EID of the result in the Scopus database
2-s2.0-85101831681