Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00425592" target="_blank" >RIV/60077344:_____/13:00425592 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/13:00395392

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0069197" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0069197</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0069197" target="_blank" >10.1371/journal.pone.0069197</a>

Result language

angličtina

Original language name

Nucleotide Excision Repair Is Not Induced in Human Embryonic Lung Fibroblasts Treated with Environmental Pollutants

Original language description

We tested response of normal human embryonic lung fibroblasts (HEL12469 cells) to treatment with benzo[a]pyrene (B[a]P) and extractable organic matter (EOM) from ambient air particles (PM2.5) collected in two Czech cities differing in levels and sourcesof air pollution. We analyzed multiple endpoints associated with exposure to PAHs: bulky DNA adducts and the nucleotide excision repair (NER) response [expression of XPE, XPC and XPA genes on the level of mRNA and proteins, unscheduled DNA synthesis (UDS)]. EOMs were collected in the winter and summer of 2011 in two Czech cities with different levels and sources of air pollution. The levels of bulky DNA adducts were highest after treatment with B[a]P, followed by winter EOMs; their induction by summer EOMs was weak. The induction of both mRNA and protein expression was observed, with the most pronounced effects after treatment with B[a]P (?S9); the response induced by EOMs from both cities and seasons was substantially weaker. The expre

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GAP503%2F11%2F0084" target="_blank" >GAP503/11/0084: The effect of polycyclic aromatic hydrocarbons and particulate matter on DNA damage and DNA repair mechanisms in vitro</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

000322391400043

EID of the result in the Scopus database

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