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The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00426076" target="_blank" >RIV/60077344:_____/14:00426076 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S1046202314000449" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1046202314000449</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ymeth.2014.02.014" target="_blank" >10.1016/j.ymeth.2014.02.014</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitly

  • Original language description

    Transcription activator-like effector nucleases (TALENs) are custom-made enzymes designed to cut double-stranded DNA at desired locations. The DNA breaks are repaired either by error-prone non-homologous end-joining (NHEJ) pathway or via homologous recombination requiring homologous DNA as a template for the repair. TALENs are used for site-specific mutagenesis in an extended range of organisms including insects. We will describe here a simple TALEN-based mutagenesis protocol suitable for the generationof germline mutations in Bombyx mori and Drosophila melanogaster. The protocol includes assembly of specific TAL modules, in vitro synthesis of TALEN RNAs, egg microinjection and mutation detection using PCR analysis. Our procedure allows a high frequency induction of NHEJ mutations, which often allows the reception of homozygous mutants already in the G1.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP305%2F10%2F2406" target="_blank" >GAP305/10/2406: Targeted mutagenesis in silkworm</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Methods

  • ISSN

    1046-2023

  • e-ISSN

  • Volume of the periodical

    69

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

    46-57

  • UT code for WoS article

    000341803500008

  • EID of the result in the Scopus database