Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00429865" target="_blank" >RIV/60077344:_____/14:00429865 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/14:00429865 RIV/60076658:12310/14:43887150
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400113/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400113/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201400113" target="_blank" >10.1002/jssc.201400113</a>

Result language
angličtina
Original language name
Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects
Original language description
The quantification of phospholipid classes and the determination of their molecular structures are crucial in physiological and medical studies. This paper?s target analytes are cell membrane phospholipids, which play an important role in the seasonal acclimation processes of poikilothermic organisms.Weintroduce a set of simple and cost-effective analytical methods that enable efficient characterization and quantification of particular phospholipid classes and the identification and relative distribution of the individual phospholipid species. The analytical approach involves solid-phase extraction and high-performance thin-layer chromatography, which facilitate the separation of particular lipid classes. The obtained fractions are further transesterified to fatty acid methyl esters and subjected to gas chromatography coupled to flame ionization detection, which enables the determination of the position of double bonds. Phospholipid species separation is achieved by high-performance li
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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