Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F14%3A00436606" target="_blank" >RIV/60077344:_____/14:00436606 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/14:43887954

Result on the web

<a href="http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf" target="_blank" >http://www.biomedcentral.com/content/pdf/1471-2156-15-S2-S15.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2156-15-S2-S15" target="_blank" >10.1186/1471-2156-15-S2-S15</a>

Result language

angličtina

Original language name

Mapping of single-copy genes by TSA-FISH in th codling moth, Cydia pomonella

Original language description

In this study, we adapted a FISH protocol with tyramide signal amplification (TSA-FISH) for detection of single-copy genes in Lepidoptera. We tested the protocol with probes prepared from partial sequences of Z-linked genes in the codling moth. Using a modified TSA-FISH protocol we successfully mapped a partial sequence of the Acetylcholinesterase 1 (Ace-1) gene to the Z chromosome and confirmed thus its Z-linkage. A subsequent combination of BAC-FISH with BAC probes containing anticipated neighbouringZ-linked genes and TSA-FISH with the Ace-1 probe allowed the integration of Ace-1 in the physical map of the codling moth Z chromosome. We also developed a two-colour TSA-FISH protocol which enabled us simultaneous localization of two Z-linked genes, Ace-1 and Notch, to the expected regions of the Z chromosome. Our results suggest that TSA-FISH represents a reliable technique for physical mapping of genes or localization of transgene cassettes on chromosomes of moths and butterflies.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

B M C Genetics

ISSN

1471-2156

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

Supplement 2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—