Imaging of tissue sections with very slow electrons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00436878" target="_blank" >RIV/60077344:_____/15:00436878 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/15:00436878

Result on the web

<a href="http://dx.doi.org/10.1016/j.ultramic.2014.10.009" target="_blank" >http://dx.doi.org/10.1016/j.ultramic.2014.10.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ultramic.2014.10.009" target="_blank" >10.1016/j.ultramic.2014.10.009</a>

Result language

angličtina

Original language name

Imaging of tissue sections with very slow electrons

Original language description

The examination of thin sections of tissues with electron microscopes is an indispensable tool. Being composed of light elements, samples of living matter illuminated with electrons at the usual high energies of tens or even hundreds of kiloelectronvoltsprovide very low image contrasts in transmission or scanning transmission electron microscopes. Therefore, heavy metal salts are added to the specimen during preparation procedures (post-fixation with osmium tetroxide or staining). However, these procedures can modify or obscure the ultrastructural details of cells. Here we show that the energy of electrons used for the scanned transmission imaging of tissue sections can be reduced to mere hundreds or even tens of electronvolts and can produce extremely high contrast even for samples free of any metal salts. We found that when biasing a sufficiently thin tissue section sample to a high negative potential in a scanning transmission electron microscope, thereby reducing the energy of the

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Ultramicroscopy

ISSN

0304-3991

e-ISSN

—

Volume of the periodical

148

Issue of the periodical within the volume

JAN 2015

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

146-150

UT code for WoS article

000345973000018

EID of the result in the Scopus database

2-s2.0-84909592404