Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00443139" target="_blank" >RIV/60077344:_____/15:00443139 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.cropro.2014.12.013" target="_blank" >http://dx.doi.org/10.1016/j.cropro.2014.12.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cropro.2014.12.013" target="_blank" >10.1016/j.cropro.2014.12.013</a>
Alternative languages
Result language
angličtina
Original language name
Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes
Original language description
Phytoplasmas are economically important pathogens of various fruit trees. Reliable techniques therefore are needed for their detection and discrimination. For this purpose, an oligonucleotide microarray targeting the ribosomal protein genes rps3, rpl22,and rps19 of phytoplasmas was tested. Following PCR (35 cycles) of total DNA from phytoplasma-infected plants with Cy5-labelled primer, the microarray reliably detected 16Sr groups I,II,III,V,VI,VII,IX,X, and XII in single infections as well as six different mixed infections (I+II,I+III, I+V, I+VII, I+IX, and I+X) prepared artificially by mixing DNA prior to PCR It also successfully classified 16Sr groups from field samples collected in the Czech Republic (16Sr groups I,III,and mixed infection I+X). Despite that it did not succeed in distinguishing another 2 artificial mixed infections (I+VI and I+XII),the microarray developed here provides a suitable alternative to rRNA-based microarrays published previously and where only single infec
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Crop Protection
ISSN
0261-2194
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
January 2015
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
47-52
UT code for WoS article
000350088200007
EID of the result in the Scopus database
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