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Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00443139" target="_blank" >RIV/60077344:_____/15:00443139 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.cropro.2014.12.013" target="_blank" >http://dx.doi.org/10.1016/j.cropro.2014.12.013</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.cropro.2014.12.013" target="_blank" >10.1016/j.cropro.2014.12.013</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Discrimination of phytoplasmas using an oligonucleotide microarray targeting rps3, rpl22, and rps19 genes

  • Original language description

    Phytoplasmas are economically important pathogens of various fruit trees. Reliable techniques therefore are needed for their detection and discrimination. For this purpose, an oligonucleotide microarray targeting the ribosomal protein genes rps3, rpl22,and rps19 of phytoplasmas was tested. Following PCR (35 cycles) of total DNA from phytoplasma-infected plants with Cy5-labelled primer, the microarray reliably detected 16Sr groups I,II,III,V,VI,VII,IX,X, and XII in single infections as well as six different mixed infections (I+II,I+III, I+V, I+VII, I+IX, and I+X) prepared artificially by mixing DNA prior to PCR It also successfully classified 16Sr groups from field samples collected in the Czech Republic (16Sr groups I,III,and mixed infection I+X). Despite that it did not succeed in distinguishing another 2 artificial mixed infections (I+VI and I+XII),the microarray developed here provides a suitable alternative to rRNA-based microarrays published previously and where only single infec

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Crop Protection

  • ISSN

    0261-2194

  • e-ISSN

  • Volume of the periodical

    70

  • Issue of the periodical within the volume

    January 2015

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

    47-52

  • UT code for WoS article

    000350088200007

  • EID of the result in the Scopus database