Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00367361" target="_blank" >RIV/60077344:_____/11:00367361 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Universal primers for plasmid detection and method for their relative quantification in phytoplasma-infected plants

Original language description

Rep gene occurring in the majority of plasmids associated with phytoplasmas was selected as a target for real-time PCR together with single-copy Tuf gene from the phytoplasma genome. Rep-specific primers amplified products from host plants infected withphytoplasmas of groups 16SrI, -II, -III, -V, -X, and -XII. Relative concentrations of plasmids ranged depending on strain from 1-2 copies to several hundred copies per each phytoplasma studied. Primers targeting the rep gene are proposed for direct PCR detection of phytoplasma plasmids from experimental periwinkle host as well as from natural plant hosts.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/QH91224" target="_blank" >QH91224: Research on methods for pathogen-free propagation material of currants</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bulletin of Insectology

ISSN

1721-8861

e-ISSN

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Volume of the periodical

64

Issue of the periodical within the volume

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Country of publishing house

IT - ITALY

Number of pages

2

Pages from-to

25-26

UT code for WoS article

000296681900010

EID of the result in the Scopus database

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