Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00444553" target="_blank" >RIV/60077344:_____/15:00444553 - isvavai.cz</a>
Alternative codes found
RIV/67179843:_____/15:00444553
Result on the web
<a href="http://dx.doi.org/10.1016/j.soilbio.2014.11.018" target="_blank" >http://dx.doi.org/10.1016/j.soilbio.2014.11.018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.soilbio.2014.11.018" target="_blank" >10.1016/j.soilbio.2014.11.018</a>

Result language
angličtina
Original language name
Tetracycline resistance genes persist in soil amended with cattle feces independently from chlortetracycline selection pressure
Original language description
Antibiotic residues and antibiotic resistance genes originating from animal waste represent environmental pollutants with possible human health consequences. In this study, we addressed the question whether chlortetracycline (CTC) residues in soils can act as selective pressure enhancing the persistence of tetracycline (TC-r) resistance genes in grassland soils receiving cattle feces. We performed a soil microcosm experiment, using 3 grassland soils with different management history, which were incubated with feces from conventionally raised dairy cows. The microcosms included treatments with a low dose (0.2 mg kg-1), a high dose (100 mg kg-1) and no CTC. The presence and abundance of TC-r genes tet(O), tet(Q) and tet(W) and the intI1 gene coding for class 1 integrase were assessed with real-time PCR after 0, 14, 28, 56 and 86 d of incubation. The genes tet(Q) and intI1 persisted in all feces-containing treatments for at least 28 d, and tet(W) and tet(0) for at least 86 d, though they
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)