Common and management-specific responses of grassland soil bacterial communities to manure and chlortetracycline amendments

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00543157" target="_blank" >RIV/60077344:_____/21:00543157 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/21:00549890

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S116455632100056X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S116455632100056X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejsobi.2021.103320" target="_blank" >10.1016/j.ejsobi.2021.103320</a>

Result language

angličtina

Original language name

Common and management-specific responses of grassland soil bacterial communities to manure and chlortetracycline amendments

Original language description

Manure application to soil affects the composition of soil bacterial communities as well as levels of antibiotic resistance genes in a manner dependent on various environmental and agricultural factors. These effects have been widely studied in arable soils, while the situation in grasslands that also receive inputs of fresh animal manure is largely unknown. Here, we focused on grassland soils under three different management types (pristine meadow, pasture, wintering pasture) from the same locality and compared the response of their bacterial communities to fresh cattle manure spiked or not with chlortetracycline. Soil microcosms were established either with soils (controls), + manure, + manure and chlortetracycline (CTC, 0.2 mg kg−1), and sampled at the same day (t0) and after 28 days (t28). Bacterial community composition was assessed by 454-pyrosequencing of 16S rRNA genes and correlations between the abundance of bacterial taxa and chosen tetracycline resistance genes (measured in our previous study) were calculated. Control soil bacterial communities at t0 differed significantly, reflecting differences in soil management history. Despite these initial differences, the responses of soil bacterial communities to cattle manure amendment showed similar trends in all three soils. Fresh cattle manure amendments significantly modified bacterial community structure in all soils at t0, increasing the relative abundance of OTUs affiliated to Clostridia and Bacilli. At 28d, all manure-amended soils showed a partial resilience, though they remained significantly different from controls. The effect of additional CTC was comparatively lower and only significant at t0 in all three soils. Several members of Firmicutes showed strong positive correlation with the abundance of tetracycline resistance genes tet(O), tet(Q) and tet(W), indicating that these taxa are potentially involved in dissemination of tetracycline resistance in manure-treated soils. In addition, this study indicated certain soil-management specific taxa, such as Geothrix and uncultured Burkholderiaceae, which could explain the previously observed differences in persistence of tetracycline resistance genes among soils.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10618 - Ecology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Soil Biology

ISSN

1164-5563

e-ISSN

1778-3615

Volume of the periodical

105

Issue of the periodical within the volume

July

Country of publishing house

FR - FRANCE

Number of pages

12

Pages from-to

103320

UT code for WoS article

000674539100005

EID of the result in the Scopus database

2-s2.0-85105773554