Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F15%3A00453402" target="_blank" >RIV/60077344:_____/15:00453402 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/15:43890198

Result on the web

<a href="http://dx.doi.org/10.1186/s13071-015-1281-8" target="_blank" >http://dx.doi.org/10.1186/s13071-015-1281-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13071-015-1281-8" target="_blank" >10.1186/s13071-015-1281-8</a>

Result language

angličtina

Original language name

Analysis of the mitochondrial maxicircle of Trypanosoma lewisi, a neglected human pathogen

Original language description

In this study, purified kinetoplast DNA from T. lewisi was sequenced using high-throughput sequencing in combination with sequencing of PCR amplicons. This allowed the assembly of the T. lewisi kinetoplast maxicircle DNA, which is a homologue of the mitochondrial genome in other eukaryotes. The assembly of 23,745 bp comprises the non-coding and coding regions. Comparative analysis of the maxicircle sequence of T. lewisi with Trypanosoma cruzi, Trypanosoma rangeli, Trypanosoma brucei and Leishmania tarentolae revealed that it shares 78 %, 77 %, 74 % and 66 % sequence identity with these parasites, respectively. The high GC content in at least 9 maxicircle genes of T. lewisi (ATPase6; NADH dehydrogenase subunits ND3, ND7, ND8 and ND9; G-rich regions GR3and GR4; cytochrome oxidase subunit COIII and ribosomal protein RPS12) implies that their products may be extensively edited. A detailed analysis of the non-coding region revealed that it contains numerous repeat motifs and palindromes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasites Vectors

ISSN

1756-3305

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

30 December 2015

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000367326000001

EID of the result in the Scopus database

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