Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F16%3A00461882" target="_blank" >RIV/60077344:_____/16:00461882 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/16:43890860

Result on the web

<a href="http://dx.doi.org/10.1093/gbe/evw140" target="_blank" >http://dx.doi.org/10.1093/gbe/evw140</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/gbe/evw140" target="_blank" >10.1093/gbe/evw140</a>

Result language

angličtina

Original language name

Species- and Strain-Specific Adaptation of the HSP70 Super Family in Pathogenic Trypanosomatids

Original language description

All eukaryotic genomes encode multiple members of the heat shock protein 70 (HSP70) family, which evolved distinctive structural and functional features in response to specific environmental constraints. Phylogenetic analysis of this protein family thus can inform on genetic and molecular mechanisms that drive species-specific environmental adaptation. Here we use the eukaryotic pathogen Leishmania spp. as a model system to investigate the evolution of the HSP70 protein family in an early-branching eukaryote that is prone to gene amplification and adapts to cytotoxic host environments by stress-induced and chaperone-dependent stage differentiation. Combining phylogenetic and comparative analyses of trypanosomatid genomes, draft genome of Paratrypanosoma and recently published genome sequences of 204 L. donovani field isolates, we gained unique insight into the evolutionary dynamics of the Leishmania HSP70 protein family. We provide evidence for (i) significant evolutionary expansion of this protein family in Leishmania through gene amplification and functional specialization of highly conserved canonical HSP70 members, (ii) evolution of trypanosomatid-specific, non-canonical family members that likely gained ATPase-independent functions, and (iii) loss of one atypical HSP70 member in the Trypanosoma genus. Finally, we reveal considerable copy number variation of canonical cytoplasmic HSP70 in highly related L. donovani field isolates, thus identifying this locus as a potential hot spot of environment-genotype interaction. Our data draw a complex picture of the genetic history of HSP70 in trypanosomatids that is driven by the remarkable plasticity of the Leishmania genome to undergo massive intra-chromosomal gene amplification to compensate for the absence of regulated transcriptional control in these parasites

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA14-23986S" target="_blank" >GA14-23986S: Emergence of pathogenicity for vertebrates: insight from ancestral bodonids and insect trypanosomatids</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome Biology and Evolution

ISSN

1759-6653

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

1980-1995

UT code for WoS article

000386368200010

EID of the result in the Scopus database

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