HfMV1 and another putative mycovirus in Central European populations of Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00475031" target="_blank" >RIV/60077344:_____/17:00475031 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/17:43912748 RIV/62156489:43510/17:43912748
Result on the web
<a href="https://www.balticforestry.mi.lt/bf/PDF_Articles/2017-23%5B1%5D/Baltic%20Forestry%202017.1_107-115.pdf" target="_blank" >https://www.balticforestry.mi.lt/bf/PDF_Articles/2017-23%5B1%5D/Baltic%20Forestry%202017.1_107-115.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
HfMV1 and another putative mycovirus in Central European populations of Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe
Original language description
The occurrence of putative, doubled-stranded (ds) viral RNA particles was investigated in the decaying ascomycetous fungal pathogen Hymenoscyphus fraxineus. In total, 134 isolates obtained from 134 common European ashes (Fraxinus excelsior and Fraxinus angustifolia) were obtained from three European countries (Austria, Czech Republic and Slovakia). Three different dsRNA bands of ca 2.2, 2.5 and 4.5 kb were confirmed in 28.4 % of the examined H. fraxineus samples. The dsRNA band of ca 2.2 kb was the most frequent, with 21.6 % occurrence, while the ca 4.5 kb band was the least abundant, with 15.7 % occurrence. Complementary, Hymenoscyphus fraxineus mitovirus 1 (HfMV1) was recorded through high-throughput sequencing of dsRNA in one Czech isolate and confirmed to occur in all isolates presenting bands of ca 2.5 and/or 2.2 kb in size using direct specific retro-transcriptase (RT) PCR. The Czech mitovirus strain contained a single ORF of 2154 nt, encoding an RNA-dependent RNA polymerase (RdRp). These results confirm the presence of HfMV1 in Central Europe and provide evidence of a potential novel mycovirus in H. fraxineus.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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