Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F17%3A43912653" target="_blank" >RIV/62156489:43410/17:43912653 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/17:00097502

Result on the web

<a href="https://doi.org/10.1080/09583157.2017.1368455" target="_blank" >https://doi.org/10.1080/09583157.2017.1368455</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

Original language description

We investigated the ability of Hymenoscyphus fraxineus mitovirus 1 (HfMV1) to spread in nature through the airborne inoculum of its fungal host, the devastating Hymenoscyphus fraxineus. Eight rotating-arm spore traps were installed to collect the airborne spores (presumably ascospores) of H. fraxineus in four ecologically different ash forests in the Czech Republic. Spore trap samples were subjected to direct DNA and RNA extractions and subsequent qPCR and mitovirus-specific reverse transcription (RT) qPCR for detection and quantification of H. fraxineus and HfMV1, respectively. The prevalence of HfMV1 was confirmed in 57% of H. fraxineus sampled inocula, showing that its occurrence is significantly correlated with its fungal host H. fraxineus. Furthermore, the ratio of mitovirus carrying spores was shown to be constant at any level of H. fraxineus infection pressure independently of the studied locality. Temperatures of 19-21oC at the time of spore trapping correlated with the highest levels of both HfMV1 and H. fraxineus. Our data confirm H. fraxineus airborne inoculum as an efficient transport means for HfMV1 in nature. Moreover, this research uses a new technology to directly study a mycovirus through its host aerospora.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10607 - Virology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biocontrol Science and Technology

ISSN

0958-3157

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

992-1008

UT code for WoS article

000410578100007

EID of the result in the Scopus database

2-s2.0-85029081410