A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00479212" target="_blank" >RIV/60077344:_____/17:00479212 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1371/journal.ppat.1006310" target="_blank" >http://dx.doi.org/10.1371/journal.ppat.1006310</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.ppat.1006310" target="_blank" >10.1371/journal.ppat.1006310</a>

Result language

angličtina

Original language name

A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei

Original language description

The kinetoplast (k), the uniquely packaged mitochondrial DNA of trypanosomatid protists is formed by a catenated network of minicircles and maxicircles that divide and segregate once each cell cycle. Although many proteins involved in kDNA replication and segregation are now known, several key steps in the replication mechanism remain uncharacterized at the molecular level, one of which is the nabelschnur or umbilicus, a prominent structure which in the mammalian parasite Trypanosoma brucei connects the daughter kDNA networks prior to their segregation. Here we characterize an M17 family leucyl aminopeptidase metalloprotease, termed TbLAP1, which specifically localizes to the kDNA disk and the nabelschur and represents the first described protein found in this structure. We show that TbLAP1 is required for correct segregation of kDNA, with knockdown resulting in delayed cytokinesis and ectopic expression leading to kDNA loss and decreased cell proliferation. We propose that TbLAP1 is required for efficient kDNA division and specifically participates in the separation of daughter kDNA networks.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS Pathogens

ISSN

1553-7374

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

23

Pages from-to

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UT code for WoS article

000402555700022

EID of the result in the Scopus database

2-s2.0-85018297037