A novel nabelschnur protein regulates segregation of the kinetoplast DNA in Trypanosoma brucei
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00603905" target="_blank" >RIV/60077344:_____/24:00603905 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17310/24:A25039N3 RIV/60076658:12310/24:43909128
Result on the web
<a href="https://doi.org/10.1016/j.cub.2024.08.044" target="_blank" >https://doi.org/10.1016/j.cub.2024.08.044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.cub.2024.08.044" target="_blank" >10.1016/j.cub.2024.08.044</a>
Alternative languages
Result language
angličtina
Original language name
A novel nabelschnur protein regulates segregation of the kinetoplast DNA in Trypanosoma brucei
Original language description
The acquisition of mitochondria was imperative for initiating eukaryogenesis and thus is a characteristic feature of eukaryotic cells.1,2 The parasitic protist Trypanosoma brucei contains a singular mitochondrion with a unique mitochondrial genome, termed the kinetoplast DNA (kDNA).3 Replication of the kDNA occurs during the G1 phase of the cell cycle, prior to the start of nuclear DNA replication.4 Although numerous proteins have been functionally characterized and identified as vital components of kDNA replication and division, the molecular mechanisms governing this highly precise process remain largely unknown.5,6 One division-related and morphologically characteristic structure that remains most enigmatic is the nabelschnur, an undefined, filament-resembling structure observed by electron microscopy between segregating daughter kDNA networks.7-9 To date, only one protein, TbLAP1, an M17 family leucyl aminopeptidase metalloprotease, is known to localize to the nabelschnur.9 While screening proteins from the T. brucei MitoTag project,10 we identified a previously uncharacterized protein with an mNeonGreen signal localizing to the kDNA as well as forming a point of connection between dividing kDNAs. Here, we demonstrate that this kDNA-associated protein, named TbNAB70, indeed localizes to the nabelschnur and plays an essential role in the segregation of newly replicated kDNAs and subsequent cytokinesis in T. brucei.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Biology
ISSN
0960-9822
e-ISSN
1879-0445
Volume of the periodical
34
Issue of the periodical within the volume
20
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
4803-4812
UT code for WoS article
001388001900001
EID of the result in the Scopus database
2-s2.0-85207472404