Cytotoxicity of Pd nanostructures supported on PEN: Influence of sterilization on Pd/PEN interface

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00479853" target="_blank" >RIV/60077344:_____/17:00479853 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22310/17:43913797 RIV/60461373:22330/17:43913797 RIV/44555601:13440/17:43892933

Result on the web

<a href="http://dx.doi.org/10.1016/j.msec.2016.09.032" target="_blank" >http://dx.doi.org/10.1016/j.msec.2016.09.032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2016.09.032" target="_blank" >10.1016/j.msec.2016.09.032</a>

Result language

angličtina

Original language name

Cytotoxicity of Pd nanostructures supported on PEN: Influence of sterilization on Pd/PEN interface

Original language description

Non-conventional antimicrobial agents, such as palladium nanostructures, have been increasingly used in the medicinal technology. However, experiences uncovering their harmful and damaging effects to human health have begun to appear. In this study, we have focused on in vitro cytotoxicity assessment of Pd nanostructures supported on a biocompatible polymer. Pd nanolayers of variable thicknesses (ranging from 1.1 to 22A nm) were sputtered on polyethylene naphthalate (PEN). These nanolayers were transformed by low-temperature post-deposition annealing into discrete nanoislands. Samples were characterized by AFM, XPS, ICP-MS and electrokinetic analysis before and after annealing. Sterilization of samples prior to cytotoxicity testing was done by UV irradiation, autoclave and/or ethanol. Among the listed sterilization techniques, we have chosen the gentlest one which had minimal impact on sample morphology, Pd dissolution and overall Pd/PEN interface quality. Cytotoxic response of Pd nanostructures was determined by WST-1 cell viability assay in vitro using three model cell lines: mouse macrophages (RAW 264.7) and two types of mouse embryonic fibroblasts (L929 and NIH 3T3). Finally, cell morphology in response to Pd/PEN was evaluated by means of fluorescence microscopy.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20501 - Materials engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials Science & Engineering C-Materials for Biological Applications

ISSN

0928-4931

e-ISSN

—

Volume of the periodical

70

Issue of the periodical within the volume

Jan

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

479-486

UT code for WoS article

000388046900059

EID of the result in the Scopus database

2-s2.0-84987984529