Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F17%3A00480444" target="_blank" >RIV/60077344:_____/17:00480444 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1159/000479179" target="_blank" >http://dx.doi.org/10.1159/000479179</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000479179" target="_blank" >10.1159/000479179</a>

Result language

angličtina

Original language name

Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization

Original language description

Whole-genome shotgun reads were analyzed to determine the repeat sequence composition in the genome of black mustard, Brassica nigra (L.) Koch. The analysis showed that satellite DNA sequences are very abundant in the black mustard genome. The distribution pattern of 7 new tandem repeats (BnSAT13, BnSAT28, BnSAT68, BnSAT76, BnSAT114, BnSAT180, and BnSAT200) on black mustard chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of BnSAT13 and BnSAT76 provided useful cytogenetic markers, their position and fluorescence intensity allowed for unambiguous identification of all 8 somatic metaphase chromosomes. A karyotype showing the location and fluorescence intensity of these tandem repeat sequences together with the position of rDNAs and centromeric retrotransposons of Brassica (CRB) was constructed. The establishment of the FISH-based karyotype in B. nigra provides valuable information that can be used in detailed analyses of B. nigra accessions and derived allopolyploid Brassica species containing the B genome. (C) 2017 S. Karger AG, Basel

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/GBP501%2F12%2FG090" target="_blank" >GBP501/12/G090: Evolution and Function of Complex Plant Genomes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cytogenetic and Genome Research

ISSN

1424-8581

e-ISSN

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Volume of the periodical

152

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

158-165

UT code for WoS article

000412412600007

EID of the result in the Scopus database

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