Expression of human mutant huntingtin protein in Drosophila hemocytes impairs immune responses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00509052" target="_blank" >RIV/60077344:_____/19:00509052 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/19:43899779
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fimmu.2019.02405/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fimmu.2019.02405/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fimmu.2019.02405" target="_blank" >10.3389/fimmu.2019.02405</a>
Alternative languages
Result language
angličtina
Original language name
Expression of human mutant huntingtin protein in Drosophila hemocytes impairs immune responses
Original language description
The pathogenic effect of mutant HTT (mHTT) which causes Huntington disease (HD) are not restricted to nervous system. Such phenotypes include aberrant immune responses observed in the HD models. However, it is still unclear how this immune dysregulation influences the innate immune response against pathogenic infection. In the present study, we used transgenic Drosophila melanogaster expressing mutant HTT protein (mHTT) with hemocyte-specific drivers and examined the immune responses and hemocyte function. We found that mHTT expression in the hemocytes did not affect fly viability, but the numbers of circulating hemocytes were significantly decreased. Consequently, we observed that the expression of mHTT in the hemocytes compromised the immune responses including clot formation and encapsulation which lead to the increased susceptibility to entomopathogenic nematode and parasitoid wasp infections. In addition, mHTT expression in Drosophila macrophage-like S2 cells in vitro reduced ATP levels, phagocytic activity and the induction of antimicrobial peptides. Further effects observed in mHTT-expressing cells included the altered production of cytokines and activation of JAK/STAT signaling. The present study shows that the expression of mHTT in Drosophila hemocytes causes deficient cellular and humoral immune responses against invading pathogens. Our findings provide the insight into the pathogenic effects of mHTT in the immune cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10620 - Other biological topics
Result continuities
Project
<a href="/en/project/GJ19-13784Y" target="_blank" >GJ19-13784Y: Unveiling the mechanism underlying the innate immune response mediated by chitinase-like proteins</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Immunology
ISSN
1664-3224
e-ISSN
—
Volume of the periodical
10
Issue of the periodical within the volume
OCT 16
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
2405
UT code for WoS article
000497330500001
EID of the result in the Scopus database
2-s2.0-85074342984