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Expression of human mutant huntingtin protein in Drosophila hemocytes impairs immune responses

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00509052" target="_blank" >RIV/60077344:_____/19:00509052 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/19:43899779

  • Result on the web

    <a href="https://www.frontiersin.org/articles/10.3389/fimmu.2019.02405/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fimmu.2019.02405/full</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fimmu.2019.02405" target="_blank" >10.3389/fimmu.2019.02405</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Expression of human mutant huntingtin protein in Drosophila hemocytes impairs immune responses

  • Original language description

    The pathogenic effect of mutant HTT (mHTT) which causes Huntington disease (HD) are not restricted to nervous system. Such phenotypes include aberrant immune responses observed in the HD models. However, it is still unclear how this immune dysregulation influences the innate immune response against pathogenic infection. In the present study, we used transgenic Drosophila melanogaster expressing mutant HTT protein (mHTT) with hemocyte-specific drivers and examined the immune responses and hemocyte function. We found that mHTT expression in the hemocytes did not affect fly viability, but the numbers of circulating hemocytes were significantly decreased. Consequently, we observed that the expression of mHTT in the hemocytes compromised the immune responses including clot formation and encapsulation which lead to the increased susceptibility to entomopathogenic nematode and parasitoid wasp infections. In addition, mHTT expression in Drosophila macrophage-like S2 cells in vitro reduced ATP levels, phagocytic activity and the induction of antimicrobial peptides. Further effects observed in mHTT-expressing cells included the altered production of cytokines and activation of JAK/STAT signaling. The present study shows that the expression of mHTT in Drosophila hemocytes causes deficient cellular and humoral immune responses against invading pathogens. Our findings provide the insight into the pathogenic effects of mHTT in the immune cells.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10620 - Other biological topics

Result continuities

  • Project

    <a href="/en/project/GJ19-13784Y" target="_blank" >GJ19-13784Y: Unveiling the mechanism underlying the innate immune response mediated by chitinase-like proteins</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Immunology

  • ISSN

    1664-3224

  • e-ISSN

  • Volume of the periodical

    10

  • Issue of the periodical within the volume

    OCT 16

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    15

  • Pages from-to

    2405

  • UT code for WoS article

    000497330500001

  • EID of the result in the Scopus database

    2-s2.0-85074342984