What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00517409" target="_blank" >RIV/60077344:_____/19:00517409 - isvavai.cz</a>

Alternative codes found

RIV/68081731:_____/19:00517409 RIV/60076658:12310/19:43899169 RIV/00216305:26310/19:PU132302

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00253-018-09584-z" target="_blank" >https://link.springer.com/article/10.1007%2Fs00253-018-09584-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-018-09584-z" target="_blank" >10.1007/s00253-018-09584-z</a>

Result language

angličtina

Original language name

What keeps polyhydroxyalkanoates in bacterial cells amorphous? A derivation from stress exposure experiments

Original language description

Polyhydroxyalkanoates (PHA) are storage polymers accumulated by numerous prokaryotes in form of intracellular granules. Native PHA granules are formed by amorphous polymer which reveals considerably higher elasticity and flexibility as compared to crystalline pure PHA polymers. The fact that bacteria store PHA in amorphous state has great biological consequences. It is not clear which mechanisms protect amorphous polymer in native granules from transition into thermodynamically favorable crystalline state. Here, we demonstrate that exposition of bacterial cells to particular stressors induces granules aggregation, which is the first but not sufficient condition for PHA crystallization. Crystallization of the polymer occurs only when the stressed bacterial cells are subsequently dried. The fact that both granules aggregation and cell drying must occur to induce crystallization of PHA indicates that both previously suggested hypotheses about mechanisms of stabilization of amorphous state of native PHA are valid and, in fact, both effects participate synergistically. It seems that the amorphous state of the polymer is stabilized kinetically by the low rate of crystallization in limited volume in small PHA granules and, moreover, water present in PHA granules seems to function as plasticizer protecting the polymer from crystallization, as confirmed experimentally for the first time by the present work.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

—

Volume of the periodical

103

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

1905-1917

UT code for WoS article

000459250200029

EID of the result in the Scopus database

2-s2.0-85059773078