Accumulation of PHA granules in Cupriavidus necator as seen by confocal fluorescence microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F16%3A00464901" target="_blank" >RIV/68081731:_____/16:00464901 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/16:00464901 RIV/00216305:26310/16:PU119408

Result on the web

<a href="http://femsle.oxfordjournals.org/content/363/10/fnw094" target="_blank" >http://femsle.oxfordjournals.org/content/363/10/fnw094</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsle/fnw094" target="_blank" >10.1093/femsle/fnw094</a>

Result language

angličtina

Original language name

Accumulation of PHA granules in Cupriavidus necator as seen by confocal fluorescence microscopy

Original language description

Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (formerly Ralstonia eutropha). The cell envelope was stained by DiD (R) fluorescent probe and PHA granules by Nile Red. Signals from both probes were separated based on their spectral and fluorescence life-time properties. During growth and PHA accumulation, bacterial cells increased their length but the width of the cells remained constant. The volume fraction of PHA granules in cells increased during PHA accumulation, nevertheless, its value did not exceed 40 vol. % regardless of the PHA weight content. It seems that bacterial cultures lengthen the cells in order to control the PHA volume portion. However, since similar changes in cell length were also observed in a PHA non-accumulating mutant, it seems that there is no direct control mechanism, which regulates the prolongation of the cells with respect to PHA granules volume. It is more likely that PHA biosynthesis and the length of cells are influenced by the same external stimuli such as nutrient limitation.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BH - Optics, masers and lasers

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

—

Volume of the periodical

363

Issue of the periodical within the volume

10

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

"fnw094:1"-"7"

UT code for WoS article

000377970800011

EID of the result in the Scopus database

2-s2.0-84971645638