The highly diverged trypanosomal MICOS complex is organized in a nonessential integral membrane and an essential peripheral module

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00519416" target="_blank" >RIV/60077344:_____/19:00519416 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/19:43901853

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.14389" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1111/mmi.14389</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/mmi.14389" target="_blank" >10.1111/mmi.14389</a>

Result language

angličtina

Original language name

The highly diverged trypanosomal MICOS complex is organized in a nonessential integral membrane and an essential peripheral module

Original language description

The mitochondrial contact site and cristae organization system (MICOS) mediates the formation of cristae, invaginations in the mitochondrial inner membrane. The highly diverged MICOS complex of the parasitic protist Trypanosoma brucei consists of nine subunits. Except for two Mic10-like and a Mic60-like protein, all subunits are specific for kinetoplastids. Here, we determined on a proteome-wide scale how ablation of individual MICOS subunits affects the levels of the other subunits. The results reveal co-regulation of TbMic10-1, TbMic10-2, TbMic16 and TbMic60, suggesting that these nonessential, integral inner membrane proteins form an interdependent network. Moreover, the ablation of TbMic34 and TbMic32 reveals another network consisting of the essential, intermembrane space-localized TbMic20, TbMic32, TbMic34 and TbMic40, all of which are peripherally associated with the inner membrane. The downregulation of TbMic20, TbMic32 and TbMic34 also interferes with mitochondrial protein import and reduces the size of the TbMic10-containing complexes. Thus, the diverged MICOS of trypanosomes contains two subcomplexes: a nonessential membrane-integrated one, organized around the conserved Mic10 and Mic60, that mediates cristae formation, and an essential membrane-peripheral one consisting of four kinetoplastid-specific subunits, that is required for import of intermembrane space proteins.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Microbiology

ISSN

0950-382X

e-ISSN

—

Volume of the periodical

OCT 2019

Issue of the periodical within the volume

112

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

1731-1743

UT code for WoS article

000488620800001

EID of the result in the Scopus database

2-s2.0-85073968937