Tissue-specific signatures in tick cell line MS profiles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00519830" target="_blank" >RIV/60077344:_____/19:00519830 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/19:43899317
Result on the web
<a href="https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-019-3460-5" target="_blank" >https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-019-3460-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13071-019-3460-5" target="_blank" >10.1186/s13071-019-3460-5</a>
Alternative languages
Result language
angličtina
Original language name
Tissue-specific signatures in tick cell line MS profiles
Original language description
BackgroundThe availability of tick in vitro cell culture systems has facilitated many aspects of tick research, including proteomics. However, certain cell lines have shown a tissue-specific response to infection. Thus, a more thorough characterization of tick cell lines is necessary. Proteomic comparative studies of various tick cell lines will contribute to more efficient application of tick cell lines as model systems for investigation of host-vector-pathogen interactions.ResultsThree cell lines obtained from a hard tick, Ixodes ricinus, and two from I. scapularis were investigated. A cell mass spectrometry approach (MALDI-TOF MS) was applied, as well as classical proteomic workflows. Using PCA, tick cell line MS profiles were grouped into three clusters comprising IRE/CTVM19 and ISE18, IRE11 and IRE/CTVM20, and ISE6 cell lines. Two other approaches confirmed the results of PCA: in-solution digestion followed by nanoLC-ESI-Q-TOF MS/MS and 2D electrophoresis. The comparison of MS spectra of the cell lines and I. ricinus tick organs revealed 29 shared peaks. Of these, five were specific for ovaries, three each for gut and salivary glands, and one for Malpighian tubules. For the first time, characteristic peaks in MS profiles of tick cell lines were assigned to proteins identified in acidic extracts of corresponding cell lines.ConclusionsSeveral organ-specific MS signals were revealed in the profiles of tick cell lines.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasites & Vectors
ISSN
1756-3305
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
MAY 6 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
212
UT code for WoS article
000467272100001
EID of the result in the Scopus database
2-s2.0-85065644829