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Tissue-specific signatures in tick cell line MS profiles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F19%3A00519830" target="_blank" >RIV/60077344:_____/19:00519830 - isvavai.cz</a>

  • Alternative codes found

    RIV/60076658:12310/19:43899317

  • Result on the web

    <a href="https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-019-3460-5" target="_blank" >https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-019-3460-5</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s13071-019-3460-5" target="_blank" >10.1186/s13071-019-3460-5</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Tissue-specific signatures in tick cell line MS profiles

  • Original language description

    BackgroundThe availability of tick in vitro cell culture systems has facilitated many aspects of tick research, including proteomics. However, certain cell lines have shown a tissue-specific response to infection. Thus, a more thorough characterization of tick cell lines is necessary. Proteomic comparative studies of various tick cell lines will contribute to more efficient application of tick cell lines as model systems for investigation of host-vector-pathogen interactions.ResultsThree cell lines obtained from a hard tick, Ixodes ricinus, and two from I. scapularis were investigated. A cell mass spectrometry approach (MALDI-TOF MS) was applied, as well as classical proteomic workflows. Using PCA, tick cell line MS profiles were grouped into three clusters comprising IRE/CTVM19 and ISE18, IRE11 and IRE/CTVM20, and ISE6 cell lines. Two other approaches confirmed the results of PCA: in-solution digestion followed by nanoLC-ESI-Q-TOF MS/MS and 2D electrophoresis. The comparison of MS spectra of the cell lines and I. ricinus tick organs revealed 29 shared peaks. Of these, five were specific for ovaries, three each for gut and salivary glands, and one for Malpighian tubules. For the first time, characteristic peaks in MS profiles of tick cell lines were assigned to proteins identified in acidic extracts of corresponding cell lines.ConclusionsSeveral organ-specific MS signals were revealed in the profiles of tick cell lines.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Parasites & Vectors

  • ISSN

    1756-3305

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    MAY 6 2019

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    12

  • Pages from-to

    212

  • UT code for WoS article

    000467272100001

  • EID of the result in the Scopus database

    2-s2.0-85065644829