Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F20%3A00536352" target="_blank" >RIV/60077344:_____/20:00536352 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fphys.2020.614552/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphys.2020.614552/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2020.614552" target="_blank" >10.3389/fphys.2020.614552</a>

Result language

angličtina

Original language name

Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera)

Original language description

Lepidoptera is amongst one of the four most speciose insect orders and ecologically very successful because of their ability to fly. Insect flight is always aerobic and exactsna high metabolic demand on the animal. A family of structurally related neuropeptides, generically referred to as adipokinetic hormones (AKHs), play a key role in triggeringnthe release of readily utilizable fuel metabolites into the hemolymph from the storage forms in the fat body. We used mass spectrometry to elucidate AKH sequencesnfrom 34 species of Lepidoptera and searched the literature and publicly available databases to compile (in a phylogenetic context) a comprehensive list of all Lepidoptera sequences published/predicted from a total of 76 species. We then used the resulting set of 15 biochemically characterized AKHs in a physiological assay that measuresnlipid or carbohydrate mobilization in three different lepidopteran species to learn about the functional cross-activity (receptor-ligand interactions) amongst the differentnbutterfly/moth families. Our results include novel peptide structures, demonstrate structural diversity, phylogenetic trends in peptide distribution and order-specificitynof Lepidoptera AKHs. There is almost an equal occurrence of octa-, nona-, and decapeptides, with an unparalleled emphasis on nonapeptides than in any insect order.nPrimitive species make Peram-CAH-II, an octapeptide found also in other orders, the lepidopteran signature peptide is Manse-AKH. Not all of the 15 tested AKHs are activenin Pieris brassicae, this provides insight into structure-activity specificity and could be useful for further investigations into possible biorational insecticide development.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA17-22276S" target="_blank" >GA17-22276S: New methods for metabolomic analysis of hardly determined metabolites</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in physiology

ISSN

1664-042X

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

DEC 17

Country of publishing house

CH - SWITZERLAND

Number of pages

24

Pages from-to

614552

UT code for WoS article

000603644600001

EID of the result in the Scopus database

2-s2.0-85098643812