Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00541478" target="_blank" >RIV/60077344:_____/21:00541478 - isvavai.cz</a>
Alternative codes found
RIV/60460709:41210/21:88950
Result on the web
<a href="https://www.cambridge.org/core/journals/parasitology/article/abs/molecular-cytogenetic-analysis-of-a-triploid-population-of-the-human-broad-tapeworm-dibothriocephalus-latus-diphyllobothriidea/8D9DE3C57EA75A303B8099EEAA202AA8" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/abs/molecular-cytogenetic-analysis-of-a-triploid-population-of-the-human-broad-tapeworm-dibothriocephalus-latus-diphyllobothriidea/8D9DE3C57EA75A303B8099EEAA202AA8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0031182021000408" target="_blank" >10.1017/S0031182021000408</a>
Alternative languages
Result language
angličtina
Original language name
Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)
Original language description
The large-sized tapeworm Dibothriocephalus latus is known as the broad or fish-borne cestode of mammals that is capable to infect humans and cause diphyllobothriosis. Recently, molecular data on D. latus has been accumulating in the literature and a complete genome sequence has been published, however, little is known about the karyotype and chromosome architecture. In this study, an in-depth karyological analysis of 2 D. latus specimens was carried out. The plerocercoids originated from a perch caught in subalpine Lake Iseo (Italy) and the tapeworms were reared in hamsters. Both specimens contained cells with a highly variable number of chromosomes ranging from18 to 27. Nevertheless, the largest portion of mitotic figures (47%) showed a number corresponding to the triploid set, 3n = 27. Accordingly, the karyotype of the analyzed specimens consisted of 9 triplets of metacentric chromosomes. Fluorescence in situ hybridization (FISH) with the 18S rDNA probe clearly demonstrated the presence of 3 clusters of hybridization signals on the triplet of chromosome 7, thus confirming the triploid status of the specimens. FISH with a telomeric (TTAGGG)n probe confined hybridization signals exclusively to the terminal chromosomal regions, supporting the earlier findings that thisnrepetitive motif is a conserved feature of tapeworm telomeres.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasitology
ISSN
0031-1820
e-ISSN
1469-8161
Volume of the periodical
148
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
787-797
UT code for WoS article
000651084600003
EID of the result in the Scopus database
2-s2.0-85106000041