Highly contiguous assemblies of 101 drosophilid genomes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F21%3A00544518" target="_blank" >RIV/60077344:_____/21:00544518 - isvavai.cz</a>
Result on the web
<a href="https://elifesciences.org/articles/66405" target="_blank" >https://elifesciences.org/articles/66405</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7554/eLife.66405" target="_blank" >10.7554/eLife.66405</a>

Result language
angličtina
Original language name
Highly contiguous assemblies of 101 drosophilid genomes
Original language description
Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-qualityngenome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10616 - Entomology

Project
<a href="/en/project/GA19-13381S" target="_blank" >GA19-13381S: Cryoprotectants and cryoprotection: assessment of candidate molecules derived from research on freeze-tolerant drosophilid fly.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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