The exact timing of microinjection of parthenogenetic silkworm embryos is crucial for their successful transgenesis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00556043" target="_blank" >RIV/60077344:_____/22:00556043 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43904985
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fphys.2022.822900/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphys.2022.822900/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2022.822900" target="_blank" >10.3389/fphys.2022.822900</a>

Result language
angličtina
Original language name
The exact timing of microinjection of parthenogenetic silkworm embryos is crucial for their successful transgenesis
Original language description
The use of parthenogenetic silkworm (Bombyx mori) strains, which eliminate the problem of recombination, is a useful tool for maintaining transgenic clonal lines. The generation of genetically identical individuals is becoming an important tool in genetic engineering, allowing replication of an existing advantageous trait combination without the mixing that occurs during sexual reproduction. Thus, an animal with a particular genetic modification, such as the ability to produce transgenic proteins, can reproduce more rapidly than by natural mating. One obstacle to the widespread use of parthenogenesis in silkworm genetic engineering is the relatively low efficiency of downstream transgenesis techniques. In this work, we seek to optimize the use of transgenesis in conjunction with the production of parthenogenetic individuals. We found that a very important parameter for the introduction of foreign genes into anparthenogenetic strain is the precise timing of embryo microinjection. Our modification of the original method increased the efficiency of transgene injection as well as the survivalnrate of injected embryos. We also provide a detailed description of the methodological procedure including a graphical overview of the entire protocol.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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