Similarities and differences in fish community composition accessed by electrofishing, gill netting, seining, trawling, and water eDNA metabarcoding in temperate reservoirs.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00559607" target="_blank" >RIV/60077344:_____/22:00559607 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/22:43905087

Result on the web

<a href="https://doi.org/10.3389/fevo.2022.913279" target="_blank" >https://doi.org/10.3389/fevo.2022.913279</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fevo.2022.913279" target="_blank" >10.3389/fevo.2022.913279</a>

Result language

angličtina

Original language name

Similarities and differences in fish community composition accessed by electrofishing, gill netting, seining, trawling, and water eDNA metabarcoding in temperate reservoirs.

Original language description

It is difficult to understand the composition and diversity of biological communities in complex and heterogeneous environments using traditional sampling methods. Recently, developments in environmental DNA metabarcoding have emerged as a powerful, non-invasive method for comprehensive community characterization and biodiversity monitoring in different types of aquatic ecosystems. In this study, water eDNA targeting fish (wf-eDNA) and four traditional fish sampling methods (electrofishing, gill netting, seining, trawling) were compared to evaluate the reliability and efficiency of wf-eDNA (vertebrate mitochondrial 12S ribosomal RNA (rRNA) as an alternative approach to assess the diversity and composition of freshwater fish communities. The results of wf-eDNA showed a consistency between the traditional sampling methods regarding species detection. However, some fish species detected using wf-eDNA assay were not detected using traditional sampling methods and vice versa. Comparison of wf-eDNA and traditional sampling methods revealed spatial homogeneity in fish community composition in all reservoirs. Ordination analysis showed that the wf-eDNA approach covers all traditional sampling methods and occupies an intermediate position. In addition, based on the Shannon diversity index, we found that in one reservoir the wf-eDNA method yielded similar fish community diversity to traditional sampling methods. However, in other reservoirs, the calculated Shannon diversity index of the wf-eDNA method was significantly higher than traditional sampling methods. In general, significant positive correlations were found between the wf-eDNA method and almost all traditional sampling methods. We conclude that wf-eDNA seems to be a reliable and complementary approach for biomonitoring and ecosystem management of freshwater ichthyofauna.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10618 - Ecology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Ecology and Evolution

ISSN

2296-701X

e-ISSN

2296-701X

Volume of the periodical

10

Issue of the periodical within the volume

Jul

Country of publishing house

CH - SWITZERLAND

Number of pages

17

Pages from-to

913279

UT code for WoS article

000827260700001

EID of the result in the Scopus database

2-s2.0-85134205289