Using the multi-omics approach to reveal the silk composition in Plectrocnemia conspersa

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00559958" target="_blank" >RIV/60077344:_____/22:00559958 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/22:00559958 RIV/60076658:12310/22:43904664 RIV/60460709:41320/22:94267

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fmolb.2022.945239/pdf" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmolb.2022.945239/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmolb.2022.945239" target="_blank" >10.3389/fmolb.2022.945239</a>

Result language

angličtina

Original language name

Using the multi-omics approach to reveal the silk composition in Plectrocnemia conspersa

Original language description

Similar to Lepidoptera, the larvae of Trichoptera are also capable of producing silk. Plectrocnemia conspersa, a predatory species belonging to the suborder Annulipalpia, builds massive silken retreats with preycapturing nets. In this study, we describe the silk glands of P. conspersa and use the multi-omics methods to obtain a complete picture of the fiber composition. A combination of silk gland- specific transcriptome and proteomic analyses of the spun-out fibers yielded 27 significant candidates whose full-length sequences and gene structures were retrieved from the publicly available genome database. About one-third of the candidates were completely novel proteins for which there are no described homologs, including a group of five pseudofibroins, proteins with a composition similar to fibroin heavy chain. The rest were homologs of lepidopteran silk proteins, although some had a larger number of paralogs. On the other hand, P. conspersa fibers lacked some proteins that are regular components in moth silk. In summary, the multi-omics approach provides an opportunity to compare the overall composition of silk with other insect species. A sufficient number of such studies will make it possible to distinguish between the basic components of all silks and the proteins that represent the adaptation of the fibers for specific purposes or environments.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10605 - Developmental biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in molecular biosciences

ISSN

2296-889X

e-ISSN

2296-889X

Volume of the periodical

9

Issue of the periodical within the volume

AUG 11

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

945239

UT code for WoS article

000848541300001

EID of the result in the Scopus database

2-s2.0-85137200525