Stabilization of insect cell membranes and soluble enzymes by accumulated cryoprotectants during freezing stress
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F22%3A00561779" target="_blank" >RIV/60077344:_____/22:00561779 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/22:43906031
Result on the web
<a href="https://www.pnas.org/doi/epdf/10.1073/pnas.2211744119" target="_blank" >https://www.pnas.org/doi/epdf/10.1073/pnas.2211744119</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1073/pnas.2211744119" target="_blank" >10.1073/pnas.2211744119</a>
Alternative languages
Result language
angličtina
Original language name
Stabilization of insect cell membranes and soluble enzymes by accumulated cryoprotectants during freezing stress
Original language description
Most multicellular organisms are freeze sensitive, but the ability to survive freezing of the extracellular fluids evolved in several vertebrate ectotherms, some plants, and many insects. Here, we test the coupled hypotheses that are perpetuated in the literature: that irreversible denaturation of proteins and loss of biological membrane integrity are two ultimate molecular mechanisms of freezing injury in freeze-sensitive insects and that seasonally accumulated small cryoprotective molecules (CPs) stabilize proteins and membranes against injury in freeze-tolerant insects. Using the drosophilid fly, Chymomyza costata, we show that seven different soluble enzymes exhibit no or only partial loss of activity upon lethal freezing stress applied in vivo to whole freeze-sensitive larvae. In contrast, the enzymes lost activity when extracted and frozen in vitro in a diluted buffer solution. This loss of activity was fully prevented by adding low concentrations of a wide array of different compounds to the buffer, including C. costata native CPs, other metabolites, bovine serum albumin (BSA), and even the biologically inert artificial compounds HistoDenz and Ficoll. Next, we show that fat body plasma membranes lose integrity when frozen in vivo in freeze-sensitive but not in freeze-tolerant larvae. Freezing fat body cells in vitro, however, resulted in loss of membrane integrity in both freeze-sensitive and freeze-tolerant larvae. Different additives showed widely different capacities to protect membrane integrity when added to in vitro freezing media. A complete rescue of membrane integrity in freeze-tolerant larvae was observed with a mixture of proline, trehalose, and BSA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
<a href="/en/project/GA19-13381S" target="_blank" >GA19-13381S: Cryoprotectants and cryoprotection: assessment of candidate molecules derived from research on freeze-tolerant drosophilid fly.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424
e-ISSN
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Volume of the periodical
119
Issue of the periodical within the volume
41
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
e2211744119
UT code for WoS article
000969668100001
EID of the result in the Scopus database
2-s2.0-85139371797